Ripon SS, Mahmood A, Chowdhury MM and Islam MT
Previous reports saying that, the shrub, Clerodenrum viscosum Vent. has a number of important biological activities. Oxidative stress and inflammatory events trigger cardiovascular diseases (CVD), known as one of the major consequences in health problems. This study evaluated a possible clotlysis activity of C. viscosum leaf (MECVL). The hot MECVL undergone for antiradical (DPPH•: 1,1-diphenyl- 2-picrylhydrazyl radical), egg albumin protection and inhibition of hemolysis of human erythrocytes (HRBCs), and clotlysis analysis, taking ascorbic acid, acetyl salicylic acid and streptokinase as standards, respectively. Additionally, a preliminary phytochemical study was also conducted, suggesting the presence of alkaloids, glycosides, flavonoids, reducing sugars, and gums in MECVL. The MECVL concentration dependently scavenged the DPPH•, inhibited protein denaturation, protected HRBCs and caused clotlysis. In the DPPH assay, MECVL at 100 μg/mL scavenged DPPH radical by 47.1 ± 0.8%. The highest activity was observed at the highest concentration of the MECVL (500 μg/mL), with which it inhibited protein denaturation, protected HRBCs and caused clotlysis by 81.5 ± 0.1%, 89.4 ± 0.1% and 81.9 ± 4.8%, respectively. Although in all cases the standards exhibited better activities, but the effects of MECVL should be considered significant (p<0.05) in comparison to the negative control. The extract exhibited an anti-atherothrombosis capacity possibly via antioxidant, anti-inflammatory and membrane stabilization pathways, recommending C. viscosum as a promising phytotherapeutic tool, especially on pathology of CVD.
