B. Minny Priyanka , Jannet Vennila. J and Ganesh. S
Kala-azar or Visceral Leishmaniasis is caused by the parasite Leishmania donovani which affects liver, spleen and bone marrow. Aloe vera is a medicinal plant which contains active components that inhibit Leishmania donovani growth. Fresh leaves of Aloe vera were collected and dried. The active components of Aloe vera were extracted by hot percolation using soxhlet apparatus. The crude methanolic extracts of Aloe vera leaves were analyzed by GC/MS. Docking of protein by active components from Aloe vera were performed by insilico method ten components were found in Aloe vera extract. The ten structures were retrieved from PubChem. In the present study, CDC42 protein was modeled by Modeller and the active components of Aloe vera are docked with the target protein CDC42. One of the active components Sitosterol was found to show interaction in par with the natural inhibitor Secramine B. Therefore it is suggested that Sitosterol in Aloe vera inhibits the promastogote formation in Leishmania donovini and thereby Aloe vera can be used to treat visceral leishmaniasis.
