G. Shiv, S. Gaherwal, C. S. Shrivastava and Nageshwar Wast
In our experiment three different types of plant species were selected (Aegle marmelos, Solanum xanthocarpum and Calotropis procera) against three different types of fungus (Aspergillus niger, Candida albicans and Phenerochaete chrysosporium) in five different organic solvents (Hexane, Benzene, Methanol, Ethanol, Petroleum ether). Only extract of Aegle marmelos and Solanum xanthocarpum in Hexane showed positive result against fungus Candida albicans and the remaining showed negative rest. To screen the antifungal activity of plant, Pour plate and agar well diffusion technique is used. In the present study, if we increase the concentration, zone of inhibition also increases. This shows that in plant extract some compounds are present which have antifungal properties. Maximum growth inhibition was found in 500 ug/ml and minimum growth inhibition found in 100 ug/ml concentration of Hexaneic extract of S. xanthocarpum and A.marmalos against Candida albicans. As a positive control we used antifungal agent like fuconazole. In quantitative analysis fungal growth are inhibited if the time period is increased. In present study, LC-MS results of plant extract showed the presence of some chemical compounds which are responsible for antifungal property. Aegle marmelos leaves contained γ-sitosterol, aegelin, lupeol, rutin, marmesinin, β-sitosterol, flavone, glycoside, Oisopentenyl halfordiol, and marmeline and Phenylethyl cinnamamide. Solanum xanthocarpum Leaf contains different type of alkaloids (solasodine, solanine) and terpenoids, which are responsible for antifungal activity.
