European Journal of Experimental Biology Open Access

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Abstract

In vitro-Propagation of Threatened African Sandalwood (Osyris lanceolata) Using Indole-3-Butyric Acid Hormone (IBA)

Adugnaw Admas*, Smegnew Melese, Amare Genetu, Zewdu Yilma, Berhane Kidane, Melaku Admasu

Osyris lanceolata is a threatened shrub endemic to East Africa and South African regions, which is being severely affected by fungi, uprooting for oil extraction, poor natural regeneration, phenological structures (dioecious), medicinal values, lack of sexual recruitment and habitat loss. It has been found a challenge through application of in situ conservation of natural trees as such since rapid human population growth and the available natural strands of valuable plants of African sandalwood have not been able to meet the demands of the people in world. However, this study via using the advances of plant propagation method it provided new options for conserving and multiplication of Osyris lanceolata species using in vitro culture techniques of by using IBA hormone. Propagation of Osyris lanceolata by IBA in non-mist poly-propagator was investigated by taking young stem of Osyris lanceoleta from Bazawit Hill and we provided an alternative propagation technique to the use of seeds. New leaf was initiated on the young stems of the Osyris after six weeks of the experiment. The influence of IBA as rooting promoter at three concentrations (50, 100 and 150 ppm) were recorded. Unexpectedly, from collected data we observed that the success 83.3% can be achieved from young stem of untreated stem by hormone and 50% were achieved in the treated plant, this propagation technique is a viable alternative to seed. The success may influenced by application of the origin stem cutting from a mother plant with a type of soil.

Published Date: 2025-01-24; Received Date: 2023-08-07