Experimental Techniques for Collecting X-Ray Diffraction Data from Protein Crystals over a Broad Temperature Range

Swapna Sanika

Abstract

Experimental Techniques for Collecting X-Ray Diffraction Data from Protein Crystals over a Broad Temperature Range

Swapna Sanika

Traditional X�?�ray diffraction facts collected at cryo�?�temperatures have introduced profitable insights into the three�?�dimensional structures of proteins, imparting the backbone of shape–function studies. While cryo�?�cooling mitigates radiation harm, cryo�?� temperatures can regulate protein conformational ensembles and solvent shape. Furthermore, conformational ensembles underlie protein function and energetics, and present day advances in room�?�temperature X�?�ray crystallography have introduced conformational heterogeneity statistics that can be right away related to natural function.