Mansour Binandeh*
The project is based on extensive studies on applied nanoparticles in biology and medicine. So initially Fe3O4 magnetic nanoparticles (MNPS) with core/shell structure of silica coating were synthesized by chemical coprecipitation method. Which were synthesized in 100 nm in size and its structure was analyzed by tools such as SEM and FT-IR. The purpose of this production was to use nanoparticles in the absorption of biomolecules DNA-L, C (linear, convoluted) and protein (BSA), So that the amount of 1 ml with an optimal concentration of 25 μgml-1 of (DNA-L, C or BSA) was solved in 20 mg MNPS at room temperature and 0.5 hr of optimal time. After sampling, the samples were analyzed by an ultraviolet spectrophotometer (UV-Vis) and electrophoresis (base for the decrease of stains). The results showed that absorption and diffusion of DNAS-BSA at the surface of nanoparticles were 85% and 65% respectively, and their release rate in buffered solutions (i.e., In 1 ml of trisaminomethane HCl and PBS buffers) was found to be about 60-80%. So after review, linkage of electrostatic bonding between nanoparticles and biomolecules was obtained and the results of the EDX analysis confirm this study. With the endured studies, this project can be a way of stabilizing biological molecules by MNPS [(DNA-L, C) or protein] systems. So hope is that, in the future this method will be used to transport targeted proteins and DNAS and repair damaged cells to be used.
