Andrey Nikolaevych Belousov
Objectives: Red blood cells (RBCs) into bags containing anticoagulant citrate, nutrient phosphate and dextrose (CPD); RBCs into bags containing anticoagulant citrate, nutrient phosphate, dextrose and adenine (CPDA-1). Methods: As membrane protective used saline which had previously been treated with magnetite nanoparticles (ICNB) by the Belousov’s method. The physiological solution that was treated with nanoparticles was added to the preserved RBCs according to the developed method. Sample of control was the addition of intact saline. IR Spectrophotometer-29 (LOMO), working in NSC Kharkov Institute of Physics and Technology of the National Academy of Sciences of Ukraine, was used for registration of absorption spectra of an aqueous solution of erythrocytes in the IR range. Results: The method of infrared spectroscopy made it possible to track the dynamics of changes in all important types of bonds in molecules of erythrocyte membranes at the stages of their storage at positive temperature. The results clearly showed that the presented method of application of nanotechnology significantly increases the storage time of RBCs in different versions of preservatives due to mechanisms to reduce violations of the molecular structure of proteins and lipids in the erythrocyte membranes. In the future, with used nanotechnologies is planned to continue to study the features of metabolic processes of preserved RBCs at storage stages at positive temperature. Conclusions: Nevertheless, today it is obvious that the presented method of application of nanotechnology is not only safe for use in practice in the Blood Service, Transfusiology and Hematology, but also is the most promising innovation project.
