European Journal of Experimental Biology Open Access

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Abstract

Microbial production of protease by Bacillus Cereus using cassava waste water

R. Santhi

The extracellular microbial proteases have several applications in various industries which account for approximately 40% of the total enzyme in the work market. In the present study, protease production from Bacillus cereus was studied and different parameters were optimized such as incubation time, pH, carbon and nitrogen were optimized. Maximum protease production was observed at 48 hours with pH 7; increase in production was observed when glucose and peptone were used as carbon and nitrogen sources in the production medium respectively. Further, cassava waste water was also used as an alternative carbon source for enzyme production, showing a maximum of protease production when supplemented with 6 % in the production medium.