R. B. Singh
Polysaccharides was isolated from crushed seeds of Wrightia tinctoria R.Br. (Roxb.) with water and precipitation with ethanol to obtained in crude form as D-galactose and D-mannose in 1:3 molar ratio, as determined by alkaline hypoiodite method. Crude polysaccharide consumed 1.36 moles of iodine by iodometrically. The obtained monosaccharides were characterized by paper chromatograph on Whatman No. 1 and 3 MM filter paper sheet and identified by column chromatography using cellulose column. The nature of linkages are of (1→4)-β-type in Dgalactopyranose and D-mannopyranose units at main polymer chain while (1→6)-α-type linkages in Dgalactopyranose at non-reducing ends which were confirmed by IR-spectra (KBr). Sugars were identified by their melting points, sulphated ash and optical rotation. Derivatives were prepared by usual manner as D-galactose phenyl hydroazone, m.p. 169-1710C, Lit mp. 170-1710C and D-mannose phenyl hydrazone m.p. 194-1950C, Lit m.p. 195-1960C. Absorbance were recorded at 814 and 874 Cm-1 region by IR-spectra (KBr.).
