European Journal of Experimental Biology Open Access
ISSN: 2248-9215
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Abstract
Production of Alkaline Protease by Bacillus pumilus (MTCC7420) using
Submerged Fermentation and Optimization of Process Parameters
O P Verma, Shruti Shukla and Abha Sigh
In the present study Bacillus pumilus (MTCC 7420) was employed for production of protease enzyme using submerged fermentation. The culture conditions were optimised for maximum enzyme production. The enzyme was stable between the pH 9.2 to 10.4 but the optimal pH was found 10.2. The enzyme was also stable between temperature ranges 40oC to 70oC but best temperature for enzyme activity was found to be 60oC. Alkaline protease activity was influenced by various carbon and nitrogen sources. The carbon and nitrogen sources were found best for fructose and gelatin respectively.
