Nilesh P. Bhosle and Sahera Nasreen
In present study the degradation potential of eight microorganisms used were Pseudomonas aeruginosa, Pseudomonas fluorescens, Bacillus cereus, Alcaligenes faecalis, Trichoderma viride, Trichoderma koningii, Penicillium chrysogenum and Rhizopus stolanifer were assessed for degradation of Cypermethrin by scale up technique under controlled environmental conditions. These four microorganisms primarily isolated from Cypermethrin treated cotton field soil and they were capable of utilizing pyrethroid pesticide Cypermethrin as sole source of carbon and energy. Bioremediation of Cypermitherin was analyzed in three varying concentration such as 50ppm, 100ppm, 150ppm in μg/ml ratio after 14 days period of incubation by dry weight of mycelia, Gas chromatography-Electronic capture detector (GC-ECD) and Gas chromatography-Mass spectroscopy (GC-MS) profiling for the metabolite detection. Simultaneously chemical oxygen demand (COD) was also carried out and recorded each 3, 7, 11, 14 days of incubation period. The GC-ECD profiling revealed that Pseudomonas auroginosa potent one to degrade the Cypermethrin 81.22%, 69.88%, 53.32% followed by Pseudomonas fluorescens 79.28%, 66.97% and 51.21% at tested 50ppm, 100ppm, 150ppm concentrations respectively after 14 days incubation period. While Bacillus cereus, Alcaligenes faecalis, Trichoderma koningii, Penicillium chrysogenum, Trichoderma viride and Rhizopus stolanifer showed moderate degradation of Cypermithrin than Pseudomonas aeruginosa. The GC-MS result sheet showed that Pathalic acid and Proponic acid are the two primary metabolites obtained. The fish tolerance also recorded with residue and Cypermethrin, result showed that fish tolerance was more in microbial degraded residue of Cypermethrin than the 100ppm Cypermethrin. Therefore Pseudomonas auroginosa was more potential to degrade the toxic compound like Cypermethrin in soil and also beneficial for soil remediation for high yield production of crops.
