Yilan Yu, Siyi Jiang, Yiou Huang, Zhongzhe Fan, Weiming Liu, Kun Wang, Wenbo Liao, Runtai Wang, Cheng Xi, Jinming Yang, Ning Huang, Xiurong Yang* and Huiyun Gan
Short Chain Chlorinated Paraffins (SCCPs) are straight-chain chlorinated hydrocarbons with carbon chain lengths between C10 and C13, which are Persistent Organic Pollutants (POPs). The health risks of SCCPs to humans and aquatic organisms are still largely unknown. Currently, the reproductive toxicity of SCCPs generally focuses on only one sex (female or male), and no comprehensive analysis comparing both female and male has been reported. The effect of SCCPs exposure on changes in gene expression in different tissues and organs remains unknown. In this study, female and male AB zebrafish were exposed to 25 μg/L, 50 μg/L, 100 μg/L, 200 μg/L and 400 μg/L SCCPs by gavage. The effect of SCCPs on the development in different tissues and organs of the offspring were assessed by the neutrophils, motor neurons and blood vessels from the hybrid progeny of the transgenic lines. In addition, we performed the RNA-seq analysis of liver, muscle and testis of adult zebrafish, including differential gene expression analysis, protein interaction prediction, enrichment analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) to fill this knowledge gap. Our study found that the effects of SCCPs on zebrafish offspring included the reduction of neutrophils (immune development suppression), blocked neuronal development (mal- formation of motor neuronal cell development) and thickened blood vessels. The results of RNA-seq provide some molecular evidence that SCCPs increase liver cancer risk, neurotoxicity and reduce sperm quality.
Published Date: 2024-02-28; Received Date: 2024-01-31
