Image - (2020) Volume 4, Issue 2
Department of Life Sciences, Kirori Mal College, University of Delhi, India
Received date: July 10, 2020; Accepted date: July 17, 2020; Published date: July 27, 2020
Citation: Kumari M (2020) Advantages of Serum-Free Media in Animal Tissue Culture. J Anim Sci Livest Prod Vol.4 No.2:6.
DCCM-1 SFM-High protein serum-free medium, designed for hybridoma cell growth and monoclonal antibody production; the culture media required for the animal tissue culture are much more complex than the minimal media required for the growth for yeasts and bacteria. In the initial years of animal tissue culture, the culture media consists of undefined components such as plasma serum and embryo extracts. But in the year 1995, Harry Eagle described the first defined media it contained salts, glucose various amino acids and vitamins which the cell cannot synthesis themselves. The serum is cellfree liquid recovered from the blood. Some common example is Fetal Bovine Serum (FBS), Calf Serum (CS) and Horse Serum (HS). The drawback of using media containing serum is that due to its undefined nature it prevents the true definition and elucidation of the nutritional and hormonal requirement of the cultured cell. But SFM, in contrast, contains defined quantities of growth factors and proteins. The quantity and quality of components in such media are precisely known; because of this, they are also called defined culture media. They provide a controlled way of study, to record the effect of specific growth factors or nutrients on cell growth and differentiation.
Figure 1: DCCM-1 SFM-High protein serum-free medium.
