Journal of the Pancreas Open Access

Keywords

Drug Therapy; Genes, BRCA1; Genes, BRCA2; Mutation; Pancreatic Neoplasms

Abbreviations

PARP: poly(ADP-ribose) polymerase

INTRODUCTION

There are approximately 40,000 new cases of pancreatic adenocarcinoma diagnosed in the USA each year [1]. It is well known that germline mutation in the breast cancer, early onset (BRCA) 1 and BRCA2 tumor suppressor genes are associated with increased risk of ovarian and breast cancer [2]. In pancreatic cancer most of the cases are sporadic but it has been reported that patients with BRCA2 mutations have 3.5 fold increase risk of developing pancreatic cancer [3]. Past series have found incidence of BRCA2 mutation as high as 17% in pancreatic cancer patients with strong family history of pancreatic cancer [4]. The association between BRCA1 and pancreatic cancer is not well defined. However one series from Ashkenazi Jews showed that BRCA1 and BRCA2 mutations are observed with nearly equal distribution in pancreas cancer families, suggesting that both genes are associated with pancreatic cancer risk [5].

What Did We Learn at the 2012 American Society of Clinical Oncol ogy (ASCO) GI Cancers Symposium?

Only one of the abstracts at the 2012 GI ASCO symposium dealt with BRCA mutation and pancreatic cancer (Abstract #217 [6]). Retrospective analysis by Tran et al. looked at Ontario Pancreatic Cancer Study and pharmacy databases and identified 5 patients with BRCA mutations (4 BRCA2 and 1 BRCA1) who were treated with platinum based regimen. It was a heterogeneous population as 3 patients received platinum based regimen for advanced disease while other 2 patients had resectable and locally advanced disease. Interestingly, patient with locally advanced disease (T4N0) were downstaged after receiving platinum based regimen and eventually underwent resection. The 3 metastatic patients had a very good response to platinum based regimen as well with progression free survival of 12 and 45 months in two of the patients.

Discussion

Patients with BRCA mutations are more susceptible certain drugs such as cisplatin or poly(ADP-ribose) polymerase (PARP) inhibitors.

Pre- clinical data suggest that platinum based regimen maybe effective in BRCA mutations at it exert their cytotoxic effect by binding directly to DNA, causing crosslinking of DNA strands and thereby inducing DNA double strand breaks. However, these damages can not repaired effectively due to lacking functioning BRCA1 or BRCA2 [7].

In breast cancer patients there are data to suggest that patients with BRCA mutations are more sensitive to DNA damaging agents such as platinum based regimen [8]. However, interestingly, in ovarian cancer it was found that BRCA2 mutation was more associated with response to platinum-based therapy when compared with BRCA1 mutation carriers [9]. In pancreatic cancer, in vitro data suggest that pancreatic cancers with BRCA2 mutations are more susceptible to DNA cross liking agents [10]. However, clinically this hypothesis is yet to be validated even though there are cases of patients responding to platinum based regimen after failing multiple lines of therapy [11, 12]. The Abstract #217 presented at the 2012 ASCO GI Cancers Symposium supports the further role of using platinum based regimen in pancreatic cancer with BRCA mutation. Even though the numbers were once again small there was evidence of activity of platinum based regimen in this highly selected subset of population.

PARP inhibitors are class of drugs which can induce cell death in tumors with mutations in certain DNA repair pathways such as the BRCA pathways of double-strand break repair [13]. Similarly to the platinum based regimen, PARP inhibitors were found to have activity in population enriched for BRCA1 and BRCA2 mutations [14]. In pancreas cancer, the largest series with BRCA mutations were published from the Memorial Sloan-Kettering Cancer Center, New York, NY, USA [15]. In this series, 15 patients with BRCA mutation pancreatic cancer were identified. Three of those patients received PARP inhibitors while 6 patients received platinum based regimen. The results were promising with median survival of 27.6 months including one patient with complete response which is rare in pancreatic cancer.

Conclusion

The incidence of BRCA mutations in pancreatic cancer is small. However, these subsets of patients are clinically important as they may potentially benefit from “targeted therapy”. The abstract presented at 2012 ASCO GI Cancers Symposium supports the hypothesis of using platinum based regimen in pancreatic cancer patients with BRCA mutations. However, the numbers are still very small and further prospective studies are need to be done to validate the potential use of platinum based regimen or PARP inhibitors in pancreatic cancer with BRCA mutations.

Conflict of interest

The authors have no potential conflicts of interest

References

1. Jemal A, Siegel R, Xu J, Ward E. Cancer statistics, 2010. CA Cancer J Clin 2010;60:277-300.
2. Narod SA, Foulkes WD. Brca1 and brca2: 1994 and beyond. Nat Rev Cancer 2004;4:665-76.
3. Cancer risks in brca2 mutation carriers. The breast cancer linkage consortium. J Natl Cancer Inst 1999;91:1310-6.
4. Murphy KM, Brune KA, Griffin C, Sollenberger JE, Petersen GM, Bansal R, et al. Evaluation of candidate genes map2k4, madh4, acvr1b, and brca2 in familial pancreatic cancer: Deleterious brca2 mutations in 17%. Cancer Res 2002;62:3789-93.
5. Stadler ZK, Salo-Mullen E, Patil SM, Pietanza MC, Vijai J, Saloustros E, et al. Prevalence of brca1 and brca2 mutations in ashkenazijewishfamilies with breast and pancreatic cancer. Cancer 2012;118:493-9.
6. Tran B MS, Zogopoulos G, Borgida A, Holter S, Gallinger S et al. Platinum-based chemotherapy (pt-chemo) in pancreatic adenocarcinoma (pc) associated with brca mutations: A translational case series. J Clin Oncol 2012;30:Abstract 217.
7. McCabe N, Lord CJ, Tutt AN, Martin NM, Smith GC, Ashworth A. Brca2-deficient capan-1 cells are extremely sensitive to the inhibition of poly (adp-ribose) polymerase: An issue of potency. Cancer Biol Ther 2005;4:934-6.
8. Byrski T, Gronwald J, Huzarski T, Grzybowska E, Budryk M, Stawicka M, et al. Pathologic complete response rates in young women with brca1-positive breast cancers after neo adjuvant chemotherapy. J Clin Oncol 2010;28:375-9.
9. Yang D, Khan S, Sun Y, Hess K, Shmulevich I, Sood AK, et al. Association of brca1 and brca2 mutations with survival, chemotherapy sensitivity, and gene mutator phenotype in patients with ovarian cancer. JAMA 2011;306:1557-65.
10. van der Heijden MS, Brody JR, Dezentje DA, Gallmeier E, Cunningham SC, Swartz MJ, et al. In vivo therapeutic responses contingent on fanconi anemia/brca2 status of the tumor. Clin Cancer Res 2005;11:7508-15.
11. Chalasani P, Kurtin S, Dragovich T. Response to a third-line mitomycin c (mmc)-based chemotherapy in a patient with metastatic pancreatic adenocarcinoma carrying germline brca2 mutation. JOP 2008;9:305-8.
12. James E, Waldron-Lynch MG, Saif MW. Prolonged survival in a patient with brca2 associated metastatic pancreatic cancer after exposure to camptothecin: A case report and review of literature. Anti-Cancer Drugs 2009;20:634-8.
13. Farmer H, McCabe N, Lord CJ, Tutt ANJ, Johnson DA, Richardson TB, et al. Targeting the DNA repair defect in brca mutant cells as a therapeutic strategy. Nature 2005;434:917-21.
14. Fong PC, Boss DS, Yap TA,Tutt A, Wu PJ, Mergui-Roelvink M, et al. Inhibition of poly (adp-ribose) polymerase in tumors from brca mutation carriers. New England Journal of Medicine 2009;361:123-34.
15. Lowery MA, Kelsen DP, Stadler ZK, Yu KH, Janjigian YY, Ludwig E, et al. An emerging entity: Pancreatic adenocarcinoma associated with a known brcamutation: Clinical descriptors, treatment implications, and future directions. Oncologist 2011;16:1397-402.