A reverse phase liquid chromatography (RP-HPLC) method has been developed and
subsequently validated for the determination of Imiquimod. Separation was achieved
with a cosmosil C18, 250 mm × 4.6 mm I.D; particle size 5 μm) Column and pH
4.6 phosphate buffer and Acetonitrile (20:80) v/v as mobile phase at a flow rate of
0.8 mL/min and the Column temperature was maintained at 25°C. UV detection
was performed at 244 nm with a run time of 10 min. The method is simple, rapid,
and selective. The described method of imiquimod is linear over a range of 5 ng/
mL to 600 ng/mL and with correlation coefficient of 0.992 respectively. The method
precision for the determination of assay was below 2.0% RSD. The method enables
accurate, precise, and rapid analysis of imiquimod.
