Journal of Food, Nutrition and Population Health Open Access

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Biological nitrogen fixation for advanced crop nutrition

3rd Edition of International Conference on Agriculture & Food Chemistry
July 23-24, 2018 Rome, Italy

Sarah Bloch

Pivot Bio, USA

Scientific Tracks Abstracts: J Food Nutr Popul Health

Abstract:

Nitrogen fertilizer has been an important part of increased grain production. Now there is a better way. Biological nitrogen fixation (BNF) by plant-associated bacteria has the potential to provide a sustainable and efficient source of nitrogen to crop roots. However, the abundance of fertilizer and residual nitrogen in agricultural soils has repressed BNF in natural rhizosphere microbes. Pivot Bio has developed a method to provide nitrogen to cereal crops by reawakening BNF in a naturally-occurring microbe. We have identified a novel bacterium that colonize the surface of corn roots at high levels and contains the genetics encoding nitrogen fixation. We fine-tuned the genetic regulation of BNF to allow the microbes to fix nitrogen in the presence of exogenous nitrogen in the lab, greenhouse and field. We also optimized the bacteria for nitrogen release to the roots, showing ammonium excretion in vitro and incorporation of biologically-fixed nitrogen into plant shoot tissues by isotopic studies. Corn plants inoculated with the resulting microbes show increased biomass in nitrogendeficient greenhouse conditions and increased yield in field conditions compared to un-inoculated controls. One of these microbes is currently in large- acre, pre-launch field trials as an in-furrow corn inoculant. By producing 28-40 kg nitrogen per hectare in corn, these microbes are the first commerciallyviable example of BNF as a nitrogen management solution, and the first step toward replacement of synthetic nitrogen fertilizers with an alternative that’s better for both growers and the environment.

Biography :

Sarah Bloch is a Senior Scientist at Pivot Bio, an agtech company based in Silicon Valley that is pioneering a ground breaking approach to crop nutrition. She spear heads the research and development of Pivot Bio’s non-transgenic microbial solutions to deliver nutrients to crops in a more sustainable and efficient manner than traditional chemical fertilizers. As Pivot Bio’s Strain Optimization Team Lead, she integrates unique strain optimization technology to improve plant growth-promoting phenotypes in plant-associated bacterial strains. She holds a PhD from the Department of Biochemistry, Molecular Biology and Biophysics from the University of Minnesota. Her graduate work focused on microbial engineering for the sustainable and low-cost production of valuable plant natural products.

E-mail: sarah@pivotbio.com