Journal of Food, Nutrition and Population Health Open Access

Changes in quality and microbial succession of lightly salted and sugar-salted blunt snout bream (Megalobrama amblycephala) fillets stored at 4°C

4^th Edition of International Conference on Agriculture & Food Chemistry

July 08-09, 2019 Vienna, Austria

Yan Li

China Agricultural University, China

Posters & Accepted Abstracts: J Food Nutr Popul Health

Abstract:

The effect of a low concentration of salt and sugar on the quality and microbial succession in blunt snout bream (Megalobrama amblycephala) fillets was assessed by sensory analysis, total volatile basic nitrogen, biogenic amines, K value, total viable counts (TVC), 16S rRNA gene analysis and Illumina MiSeq PE300 high-throughput sequencing. Fish samples were left untreated (control), treated with 1.8% salt (T1) or treated with 1.8% salt plus 0.9% sugar (T2). Consequently, salted and sugar salted treatments extended the shelf life of bream fillets by 2 days, which retarded the increase of total volatile basic nitrogen, putrescine, cadaverine and total viable counts. The putrescine and cadaverine concentrations of T2 were significantly (P<0.05) higher than T1 after day 10. Brachybacterium spp. was the major initial microbiota of bream fillets. As storage time progressed, shifts in the microbial community and lower diversity of microbiota were observed. Pseudomonas spp. and Shewanella spp. were major genera in the spoiled control group. Pseudomonas spp., Shewanella spp., and Pseudoalteromonas spp. became the main spoilers in the T1 and T2 groups. The top three dominant species Pseudomonas versuta (16/84), Shewanella putrefaciens (12/84) and Aeromonas sobria (11/84) were used and inoculated in sterile flesh blocks. The initial TVC value of inoculated groups were within 4.5-5 log CFU/g and showed a significant increase during storage, especially for the group inoculated with Pseudomonas versuta (from 4.49 to 9.59 log CFU/g), while the control group was below 2.0 log CFU/g all the time. Pseudomonas versuta was observed a strong corruption capacity of sarcoplasmic degradation and cadaverine accumulation; Shewanella putrefaciens exhibited a strong corruption capacity of myofibrillar degradation and putrescine accumulation; Aeromonas sobria could produce cadaverine and putrescine.

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