Journal of Infectious Diseases and Treatment Open Access

Clonal analysis and virulence-associated traits of native Escherichia coli from urethra of gilts and natural/artificial pregnant sows

Joint Event on 10^th Euro-Global Conference on Infectious Diseases & 5^th International Conference on Histopathology & Cytopathology

September 27-29, 2018 Rome, Italy

Sergio E Pasteris, Andrea Torres Luque, Alfredo A Martin and M V Maria C Otero

INSIBIO-UNT-CONICET, Argentina FAZ-UNT, Argentina

Posters & Accepted Abstracts: J Infec Dis Treat

Abstract:

Statements of the Problem: The reproductive performance of sows is a key factor in the herd´s productivity. Urinary tract infections (UTI) are a common problem in females, causing repeat breeding with a delayed return to estrus, which reduces the animal´s welfare and the litter performance; Escherichia coli being associated to these infections. Diverse studies described a unique microbiota in the UT in bitch. Others authors concluded that the composition of the UT bacterial communities could have an important role in the health condition of the host. Methodology: We performed the isolation and clonal association (rep-PCR, Box and Eric primers) of E. coli from the urethral microbiota of: healthy gilts-HG (n=9) and pregnant sows by natural breading-NB (n=11) or artificial insemination-AI (n=11). Also, 12 virulence factors relevant for pyelonephritic strains were evaluated by PCR: hlyA, cnf, ibeA, iutA, kpsMT II, FimH, papC, sfa/focD, afa/draBC, traT, agn43, csgA. Findings: Cultures revealed a slightly minor count (CFU/mL) for AI (3.7±0.59) group compared to HG (4.2±0.24) and NB (4.3±0.44). However, there were no differences for E. coli isolation (CFU/mL): 1.45±1.36, 2.87±1.53 and 2.61±1.84, for AI, NB and HG, respectively. The clonal analysis with both, Box or Eric primers, revealed a high similarity (>90%) between E. coli isolates from different animal groups. Positive reaction was found for: FimH (76%), agn43 (92%), traT (32%) and csgA (72%), these last ones showed a differential prevalence and were associated with E. coli from NB sows. Conclusion & Significance: These results indicate that the management conditions could affect the characteristics of the urethral microbiota in sows and, therefore, the risk for urinary tract diseases.

Biography :

Sergio E Pasteris has his expertise in the study of lactic acid bacteria (LAB) metabolism as well as in the isolation and evaluation of its beneficial properties to design probiotics for amphibian culture. Taken into account the development of resistant bacteria, some LAB strains represent an alternative instead chemotherapeutics to prevent epizootics in bullfrog systems breeding. Since bullfrog is a carrier of the etiological agent of chytridiomycosis, probiotics by using native LAB from bullfrog skin are being developed to be applied during the ex situ breeding of endangered amphibian species.

E-mail: pasteris@fbqf.unt.edu.ar