Israa M S Al-Kadmy, Sahira Nsayef Muslim, Nadheema Hammood Hussein,Alaa Naseer Mohammed Ali, Sarah Naji Aziz and Samah N El-shafeiy
Mustansiriyah University, Iraq PPRI - Agricultural Research Center, Egypt University of Plymouth, UK
Posters & Accepted Abstracts: Arch Chem Res
The production of biodiesel has increased significantly in recent years because of the need for an alternative fuel which endows with biodegradability, low toxicity and renewability. Plant oils have been employed as feedstocks for biodiesel production by transesterification process. In this work gyrB gene for Cronobacter dublinensis was sequenced and submitted to the Gen Bank databases under accession No: KY991367.1 for confirming the diagnosis as a housekeeping gene. We screened Cronobacter dublinensis isolates to produce biofuel from vegetable oil and others fats and found that the castor oil was the best among all the used oils for induction of lipase production in the medium. The dendrogram of lipase production in plates was drawn. Lip gene for lipase was sequenced and submitted to the Gen Bank databases under accession No. MF926637.1 for conform. The lipase was purified by using two-step purification procedure consisting of ethanol precipitation and ion exchange chromatography with a yield of 48.3%. The lipase has an important role in enhancement of disinfectant activity. The lipase enzyme was also used as a biocatalyst for the transesterification reaction step in biodiesel production and found that the olive oil was more suitable than the almond oil also the (palmitic acid methyl ester) was present in the major amount which is best suited for biodiesel production. We observed also that processing of the bacterial supernatants increased the performance in comparison to the nonprocessed samples since an easy and cheap process of concentration and dialysis could increase the yield of biodiesel production.
Recent Publications
1. Muslim S N, AL-Kadmy I M S, Auda I G, Mohammed-Ali A N and Al-Jubori S S (2018) A novel genetic determination of a lectin gene in Iraqi Acinetobacter baumannii Isolates and use of purified lectin as an antibiofilm agent. Journal of AOAC International 101(5):1623-1630.
2. Muslim S N, AL-Kadmy I M S, Mohammed-Ali A N, Khazaala S S, Ibrahima S A, Al-Saryia N A, Al saadia L G, Muslimb S N, Salmanc B K and Aziz S N (2018) Screening, nutritional optimization and purification for phytase produced by Enterobacter aerogenes and its role in enhancement of hydrocarbons degradation and biofilm inhibition. Microbial Pathogenesis 115:159–167.
3. AL-Kadmy I M S, A N M Ali, I M A Salman and S S Khazaal (2018) Molecular characterization of Acinetobacter baumannii isolated from Iraqi hospital environment. New Microbes New Infect. 21: 51–57.
4. Muslim S N, AL-Kadmy I M S, Mohammed-Ali A N, Salmanc B K, Ahmad M, S S Khazaal, Hussein N H and Muslim S N (2018) Chitosan extracted from Aspergillus flavus shows synergistic effect, eases quorum sensing mediated virulence factors and biofilm against nosocomial pathogen Pseudomonas aeruginosa. International Journal of Biological Macromolecules 107:52–58.
Israa M S AL-Kadmy has completed her BSc and MSc degree in Microbiology from University of Mustansiriyah, College of Science, Iraq. Academic visitor | Marine building| Faculty of science & Engineering| Plymouth University| Drake Circus | Plymouth | Devon
E-mail: israa.alkadmy@plymouth.ac.uk
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