Amira S Habib, Hazem M Warda, Ahmed Wagdi, Amany Elshorbagy and Ahmad R Bassiouny
Alhyatt Heart and Vascular Center, Egypt Tanta University Hospital, Egypt Institute of Pharmacology and Toxicology-University Medical Center Goettingen, Germany University of Alexandria, Egypt
Posters & Accepted Abstracts: Biochem Mol biol J
Objective: Syncope is a common clinical problem challenging both cardiologists and general practitioners. In our study, we selected a number of circulating micro-RNAs and vasoactive amine metabolites to be evaluated in attempts to introduce possible biomarkers for syncope. To the best of our knowledge, this is the first study to assess the changes in mi-RNAs in syncope patients.
Materials & Methods: Nineteen patients with history of syncope and nineteen sex and age matched healthy controls participated. A detailed medical history was recorded and cardiovascular examinations followed by head up tilt table testing (HUTT) were performed. Three blood samples were withdrawn, first one at baseline, second during syncopal attack and third one after 30 minutes from the end of the tilt test. The levels of three circulating microRNAs (miR-210, miR-1 and miR-34a) and three vasoactive amine metabolites (endothelin-1, copeptin and serotonin) were quantified.
Results: In Group A, copeptin significantly increased during syncopal attack by 21.7±0.45 pg/mL vs. 4.3±1.209 pg/mL in control subjects (Group B; P=0.002). Similarly, endothelin-1 values significantly rose by an average of 28±1.25 pg/mL in syncope patients vs. 3.35±0.75 pg/mL in healthy controls (P<0.001). Serotonin (5-HT) levels were significantly greater during syncope relative to baseline in HUTT positive patients by 95.89±3.7 pg/mL vs. 9±1.43 pg/ml (p<0.001) in control subjects. In summary, vasoactive amines increased with a 3-5 fold change in group A, but showed 1-2 fold increase in the control group. In group A, miR-210 has increased by a mean of 0.6±0.09 (p<0.001) during syncope (95% CI [0.4-0.79]). While miR-34a values increased by mean of 0.89±0.22 during syncope than baseline value (95% CI [0.42, 1.36]) with significant difference of P=0.001. Likewise, miR-1 levels was elevated by an average of 0.42±0.07 (95% CI [0.26, 0.58]) with a p<0.001 significance. miRNA levels were 3±1 fold higher in the syncope patients (Group A) than in controls.
Conclusion: The selected miRNAs and vasoactive amines have a very promising diagnostic and therapeutic potential as biomarkers in diagnosing syncope.
E-mail:
ahabib@alhyatt.com
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