Journal of Infectious Diseases and Treatment Open Access

Interleukin-36 expression in vitiligo skin lesions and its correlation with disease pattern, activity and severity

Joint Event on 10^th Euro-Global Conference on Infectious Diseases & 5^th International Conference on Histopathology & Cytopathology

September 27-29, 2018 Rome, Italy

Laura del Barrio Prieto, Irene Beitia Rodriguez, Laura Martinez Codes, Mar Olmo Fernandez, M Jes�ºs Lago Perez and Blanca Palomeque Castro

Hospital Del Henares, Spain

Posters & Accepted Abstracts: J Infec Dis Treat

Abstract:

Introduction: Macrocassettes are a very useful tool in the pathology laboratory. Currently, they are usually carried out exclusively in radical prostatectomies. We are pioneers in Spain in using them for all types of tissues. It is not usually known the adequate processing that allows the realization of good quality slides in any type of tissue. The fact that the pathologist can visualize complete structures or larger lesions in a single slide improves efficiency. Purposes: To present our experience for more than ten years in the fixation, processing, inclusion and cutting of numerous biopsies in macrocassettes (more than 4000) and show the advantages and disadvantages of the use of this technique. Currently, we have launched a practical workshop for pathology technicians teaching these techniques. In this first edition of the workshop we have full occupation from the participants and also we have received numerous emails from professionals from outside Spain to get information to participate. Equipment & Methods: For more than ten years we have made macrocassettes of all kinds of tissues, bone, kidney, colon, breast, brain with fixings and different processing times, processing the macrocassettes separated from the routine biopsies (due to the time of permanence in each liquid), until obtaining optimal results in each type of tissue. We present our processing protocols and abundant iconography of the process and the results. Results & Conclusion: The use of macrocassettes facilitates the diagnosis by the pathologist to be able to visualize structures in a more complete way, provide more accurate tumor volumes and more appropriate assessment of surgical edges, automated immunohistochemistry can be performed, collecting from the flotation bath the area that is needed for diagnosis. This technique increases the profitability in the daily work by decreasing the number of cassettes. It’s recommended to incorporate this technique to the routine only with a specific processor and qualified technicians in the handling of this type of cassettes.

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