Archives in Chemical Research Open Access

On-line and off-line analysis using a microfluidic-chip as sample treatment miniaturization

7^th Edition of International Conference and Exhibition on Separation Techniques

July 05-07, 2018 Berlin, Germany

Maria Ramos Payan, Rut Fernandez Torres and Miguel Angel Bello Lopez

University of Sevilla, Spain

Posters & Accepted Abstracts: Arch Chem Res

Abstract:

Statement of the Problem: The most critical stage of the analytical process is the preparation of the sample requiring different stages prior to analysis, long extraction times, large volumes of reagents, etc., with the objective of obtaining a good clean-up for the analysis by instrumental techniques (as HPLC). Nowadays, one of the dominant trends in sample preparation is miniaturization and automation. In this paper, we present the advantages presented by the use of microfluidic systems in this field. These devices allow to work in different configuration s depending on the nature of the analyte to be extracted: either by liquid phase microextraction or by electromembranes. Methodology: Our group have demonstrated the possibility of on-line and off-line analysis by HPLC. Two pumps are used to introduce the sample and the acceptor phase into the microfluidic device. The microfluidic device is fabricated using two patterned plates of poly(methyl methacrylate), which are symmetrical. The channels are separated by a polypropylene membrane. For offline analysis, the acceptor outlet (extract) is collected and injected directly into a HPLC. For on-line analysis, the acceptor outlet is connected to the HPLC. Findings: This type of device provides high selectivity, cleanup, reduces sample volume and low consumption of reagents, significantly reduces time of analysis and has demonstrated its ability to online coupling with HPLC. Additionally, the microchip-devices are reusable (allow membrane exchange) and each membrane is stable during more than ten consecutive microextractions. Conclusion & Significance: The miniaturization and automatization of sample treatment procedures (on-chip) offer multiple advantages compared with existing traditional techniques. It also, offer excellent clean-up either with biological or environmental samples and significantly reduce the time of analysis from the sample collection till data obtaining. Recent Publications 1. Maria Ramos Payan; Santiago Maspoch; Andreu Llobera. An effective microfluidic based liquid-phase microextraction device (μLPME) for extraction of nonsteroidal anti-inflammatory drugs from biological and environmental samples. Talanta 165, 496 - 501. 2. Maria Ramos Payan; Santiago Maspoch; Andreu Llobera. A simple and fast Double-Flow microfluidic device based liquid-phase microextraction (DF-μLPME) for the determination of parabens in water samples. Talanta 165, 496-501 3. María Ramos Payán; Henrik Jensen; Nicolaj Petersen; Steen Honorée; Sig Pedersen Bjeergard. Liquid-phase Microextraction in a microfluidic-chip – high enrichment and sample clean-up from small volumes of biological fluids.Analytica Chimica Acta 735, 46 4. Bin Li; Nickolaj Petersen; María Ramos Payán; Steen Honorée; Stig Pedersen Bjergaard. Automated on-line clean-up and enrichment of opium alkaloids using a liquid-phase microextraction-chip directly coupled to high-performance liquid chromatography.Talanta 120, 224 - 229. 5. María Ramos Payán; Bin Li; Henrik Jensen; Nickolaj Petersen; Steen Honoreé; Stig Pedersen Bjergaard. Nano-Electromembrane

Biography :

María Ramos Payán has completed her PhD from University of Seville, Spain and postdoctoral studies from University of Copenhagen (Denmark), University of North Carolina (USA) and Microelectronic National Center of Barcelona (Spain). She is leader of the microfluidic research line. She has published more than 30 papers in reputed journals and has been serving as an editorial board member of repute.

E-mail: ramospayan@us.es