Biochemistry & Molecular Biology Journal Open Access

Overexpression of AtPAP18 gene in soybean hairy roots mediated by Agrobacterium rhizogenes

EuroScicon Joint event on Genetics, Cell and Gene Therapy

August 20-21, 2018 Amsterdam,Netherlands

Ali Izadi-Darbandi, Mehdi Younessi-Hamzekhanlu, Mohammad Ali Malboubi, Mohsen Ebrahimi, Moslem Abdipour, Francesca Sparvoli and Dario Paolo

University of Tehran, College of Aburaihan, Iran Ahar Faculty of Agriculture, University of Tabriz, Iran National Institute of Genetic Engineering and Biotechnology, Iran Jehadi-Agriculture Research Institute, Iran Biotecnologia Agraria Dipartimento di Scienze Bio-Agroalimentari Consiglio Nazionale delle Ricerche, Italy

Posters & Accepted Abstracts: Biochem Mol biol

Abstract:

Low-P stress is a challenging factor in limiting plant development. Soybean is cultivated in soils often low in phosphorus. However, on average 65% of total P arises as organic phosphates, which they become unavailable to plants unless hydrolyze to release inorganic phosphate. One approach for enhancing crop P acquisition from organic P sources is boosting activity of acid phosphatases (APases). This study seeks to understand and explain the role of an Arabidopsis (Arabidopsis thaliana) purple APase gene (AtPAP18) in soybean. Thus, the gene was isolated and final vector (AtPAP18/pK7GWG2D) was built by using Gateway instruction. Composite soybean plants were created using Agrobacterium rhizogenes mediated transformation. A. rhizogenes K599 carrying the AtPAP18/pK7GWG2D vector, carrying EGFP gene as a reporter gene, was used for soybean hairy root transformation. Analysis of EGFP expression detected fluorescence signals in transgenic roots, whereas there was no detectable fluorescence in control hairy roots. Enzyme assay results showed that the APase activity increased by 2-fold in transgenic hairy roots. The transformed hairy roots displayed meaningful increase in plant soluble P and total P contents, as compared with control plants, leading to improved biomass production. RT-PCR analysis revealed high expression levels of AtPAP18 in transformed hairy roots. It is noteworthy that these primers amplified no PAP18 transcript in control hairy roots. Taken together, it is evidently clear from the findings that overexpression of AtPAP18 gene offers an operative tactic to reduce the utilization of Pi fertilizer through increased acquisition of soil Pi, especially improving crop yield on soils low in available P.

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