Animal Cell culture is in vitro maintenance and propagation of isolated cells tissues or organs in an appropriate artificial environment. Many animal cells can be induced to grow outside of their organ or tissue of origin under defined conditions when supplemented with a medium containing nutrients and growth factors. In a cell culture technique, cells are removed from an animal and grown subsequently in a favourable environment. For animal cell culture the cells are taken from the organ of an experimental animal. The cells may be removed directly or by mechanical or enzymatic action. The cells can also be obtained by previously made cell line or cell strain.