The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, 450014, China
Research Article
Differential Transcriptome Analysis of Dunaliella Salina during Flagellar Assembly
Author(s): Liqiang Zhu, Lina Hu, Aifang Li, Shuxuan Li, Yalan Li, Qianqian Wang, Yu Huang, Yanxia Feng, Qinghua Li and Shuying Feng*
Background: Eukaryotic flagellum is highly conserved in basic structure and biogenesis, and defects in ciliary assembly or function lead to a wide range of human disease symptoms. The alga Dunaliella salina (D. salina), provides an excellent model for investigating flagellar/ciliary system. However, the genome it carries is unpublished. Results: To In this study, using high-throughput illumina RNA sequencing, the transcriptomes from flagella-assembling D. salina were analyzed firstly at an unprecedented depth. About 4 giga bases of raw sequence data were generated and 197,295 uni-genes were annotated with gene descriptions, conserved protein domains, or gene ontology terms against public databases. Among the annotated uni-genes, 25,412 uni-genes were differentially expressed during flagella regeneration, including 9,988 up-regulated uni-gene.. View More»
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