Department of Parasitology, Insect Vectors Institute Pasteur Paris, Paris, France
Research Article
Genotyping of S and C Mutated Beta Globin Gene: Development of a Set of Primers for Use with Different PCR Systems
Author(s): Koui Tossea A Stephane, Ernest Sery Gonedele-Bi, Eric Gbessi Adji, Albert A Gnondjui, Berenger Ako Ako, Coulibaly Baba, Offianan A Toure, Ibrahima Sanogo and Ronan Jambou*
Sickle cell disease is a genetic disorder that affects nearly 5% of world population. In ivory coast, SCD
is a real problem of health and screening is not systematic after born. Here, we designed a set of
primers to detect abnormal hemoglobin S and C which can be used both in conventional and
quantitative PCR (by curves combinations analysed). A total of 60 blood samples including 13 AA, 23
AS, 9 SS, 12 SC and 3 CC hemoglobin type were screened using hemoglobin electrophoresis and PCR.
The universal control primer HBU/R4 was successfully amplified for all of 60 samples. In conventional
PCR, for typing of allele S sensibility and specificity of primers were respectively 86.36% and 87.50%.
For allele C, sensibility and specificity of this pair were respectively 53.33% and 91.11%. In qPCR,
specificity and sensitivity of primers were greater than 85% for allele S a.. View More»
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