Department of Experimental Morphology, Pathology and Anthropolog, Bulgarian Academy of Sciences, Sofia, Bulgaria
Research Article
Development of Methods about Safe Application of Viral Strains with DNA-Genome as a Material for Design of New Molecular Vaccines against SARS-CoV-2/COVID-19
Author(s): Iskra Sainova*, Vera Kolyovska, Tzvetanka Markova, Dimitrina Dimitrova-Dikanarova, Desislava Drenska and Dimitar Maslarov
Taking in consideration the risk of thrombs by SARS-CoV-2 Spike (S) protein and of amyloid brain
plaques by its Nucleocapsid (N) protein, design of molecular (DNA, RNA and/or protein) vaccines
against viral Envelope (E) protein or viral Membrane (M) protein, and boosting by siRNAs against
virus genes, coding proteins S and N. Laboratory-incubated mammalian cells were transfected by
recombinant gene construct. This vector and separate sub-populations of transfected cells,
contained additional gene copy, demonstrated safety and capability for generation of adequate
immune response in vitro and in vivo. Other mammalian in vitro-cellular cultures were inoculated
with low infectious titers (high viral suspensions dilutions) of vaccine avipoxviral strains (Poxviridae
family), freezed in the presence of cryo-protector Dimethylsulfoxide (DMSO.. View More»
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